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Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells
The extent to which lipids in biological membranes self-organise into nanodomains is a subject of debate. Honigmann et al.combine scanning FCS and STED microscopies to monitor lipid diffusion over wide areas, and find that local trapping of sphingolipids may not depend on phase separation.
- Alf Honigmann
- , Veronika Mueller
- & Christian Eggeling
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Article
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A general strategy for developing cell-permeable photo-modulatable organic fluorescent probes for live-cell super-resolution imaging
Single-molecule localization microscopy depends on the use of photo-modulatable fluorescent probes; however, many cannot be used in live-cell studies due to poor cell permeability. Pan et al.present a strategy for constructing cell-permeable probes and use it to image actin filament dynamics and lysosomes.
- Deng Pan
- , Zhe Hu
- & Yu-Hui Zhang
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An authentic imaging probe to track cell fate from beginning to end
The availability of tracers to track the health of cells over long periods of time will be of value to optimize cell-based therapy. Here, Lee et al.design a nanoparticle that fluoresces red in living cells, but fluoresces green when cells begin to die from apoptosis or necrosis.
- Seung Koo Lee
- , Luke J. Mortensen
- & Ching-Hsuan Tung
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Magneto-fluorescent core-shell supernanoparticles
Magneto-fluorescent nanoparticles hold promise for bioimaging applications, but synthesizing uniform particles with tunable sizes remains challenging. Chen et al. propose an approach for co-assembling magnetic particles with fluorescent quantum dots, leading to well-defined core-shell structures.
- Ou Chen
- , Lars Riedemann
- & Moungi G. Bawendi
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Article
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Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space
Protein–protein interactions are ubiquitous in cells and these contacts are crucial for a wide number of cellular processes. Here, the authors present a technique for the super-resolution imaging and tracking of protein–protein interactions in cells.
- Zhen Liu
- , Dong Xing
- & Yujie Sun
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Separating NADH and NADPH fluorescence in live cells and tissues using FLIM
NAD and NADP play fundamentally different roles in cellular metabolism, and yet these pyridine nucleotides cannot be distinguished spectroscopically in living cells. Blacker et al.demonstrate that fluorescence lifetime imaging can be used to quantify NADPH/NADH balance in cultured cells and in the mammalian cochlea.
- Thomas S. Blacker
- , Zoe F. Mann
- & Michael R. Duchen
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Article
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Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle
Current methods for detecting proliferation in live cells cannot distinguish between dividing cells and cells that are progressing through the cell cycle. Here, a method is described that detects anillin in the contractile ring and in the midbody of cells during M-phase, providing a more accurate detection of dividing cells.
- Michael Hesse
- , Alexandra Raulf
- & Bernd K. Fleischmann
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Three-dimensional imaging of single nanotube molecule endocytosis on plasmonic substrates
Imaging and tracking the motion of single molecules on cell plasma membranes requires high spatial resolution in three dimensions. Honget al. develop a plasmonic ruler based on the fluorescence enhancement of carbon nanotubes on a gold plasmonic substrate, allowing the observation of nanotube endocytosis in three dimensions.
- Guosong Hong
- , Justin Z. Wu
- & Hongjie Dai
Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging