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| Open AccessIn vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter
Bioluminescent imaging of bacteria in vivo generally requires the use of engineered bacteria. Here the authors take advantage of bacteria specific ATP-binding cassette sugar transporters to enable selective delivery of bioluminescent nanoprobes into bacteria for visualisation, including in deep tissues.
- Qian Zhang
- , Bin Song
- & Yao He
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| Open AccessA luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice
Bioluminescence imaging of neuronal activity is desired. Here the authors report a luciferase prosubstrate activatable in vivo by nonspecific esterase and engineer a bioluminescent indicator with responsiveness to calcium ions: integration of these components enabled imaging of neuronal activity in mice.
- Xiaodong Tian
- , Yiyu Zhang
- & Hui-Wang Ai
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| Open AccessEpigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice
Here the authors show that a high-fat diet in pregnant mice can release silencing of the imprinted Dlk1 locus in multiple generations of offspring. They found that this occurs via changes in microRNA expression at the locus of interest, as well as transcriptional changes across the genome, in the developing oocytes.
- Mathew Van de Pette
- , Andrew Dimond
- & Amanda G. Fisher
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| Open AccessPortable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals
Bioluminescence imaging tends to rely on transgenic luciferase-expressing cells and animals. Here the authors report a portable bioluminescent system to non-invasively measure intra- and extracellular enzymes in vivo in non-transgenic animals which do not express luciferase.
- Aleksey Yevtodiyenko
- , Arkadiy Bazhin
- & Elena A. Goun
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| Open AccessNIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
Conventional bioluminescence imaging usually operates in the visible region and its performance is limited by strong tissue absorption and scattering. Here, the authors present bioluminescence probes (BPs) with emission in the second near infrared (NIR-II) region, and show the NIR-II-BPs could sensitively recognize tumor metastasis with a high tumor-to-normal tissue ratio.
- Lingfei Lu
- , Benhao Li
- & Fan Zhang
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| Open AccessTurn-on chemiluminescence probes and dual-amplification of signal for detection of amyloid beta species in vivo
Detection of amyloid beta deposits is often performed with fluorescent compounds that bind plaques. Here the authors develop turn-on chemiluminescent probes that bind amyloid beta plaques in vivo, and amplify the signal via chemiluminescence resonance energy transfer to the plaque-binding fluorescent molecule CRANAD-3.
- Jing Yang
- , Wei Yin
- & Chongzhao Ran
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Article
| Open AccessIn vivo non-invasive monitoring of dystrophin correction in a new Duchenne muscular dystrophy reporter mouse
Dystrophin-deficient mice are used to test corrective strategies for Duchenne muscular dystrophy, but evaluation of dystrophin expression requires collection of tissue samples from specific muscles and time points. Here, the authors generate mice in which dystrophin expression is coupled to luciferase, and show that bioluminescence allows non-invasive monitoring of dystrophin expression following genome editing.
- Leonela Amoasii
- , Hui Li
- & Eric N. Olson
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| Open AccessClick beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging
Red-shifted bioluminescence emission is needed to improve deep tissue imaging resolution. Here, the authors develop a click beetle red luciferase mutant and two naphthyl-luciferin substrates, and show the ability of the new luciferin/luciferase pairing for deep tissue multispectral tomography in mice.
- Mary P. Hall
- , Carolyn C. Woodroofe
- & Laura Mezzanotte
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| Open AccessBioluminescence imaging of G protein-coupled receptor activation in living mice
G protein-coupled receptors are involved in numerous physiological functions, thus, they represent potential pharmaceutical targets. Here Kono et al. describe a new mouse model to image GPCR activation in real-time by exploiting firefly split luciferase fragment complementation that can be detected by bioluminescence imaging.
- Mari Kono
- , Elizabeth G. Conlon
- & Richard L. Proia
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| Open AccessFive colour variants of bright luminescent protein for real-time multicolour bioimaging
The use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent proteins, and show their utility as Ca2+indicators.
- Kazushi Suzuki
- , Taichi Kimura
- & Takeharu Nagai
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| Open AccessCoupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.
- Jie Yang
- , Derrick Cumberbatch
- & Carl Hirschie Johnson
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| Open AccessMonitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET) that allows efficient real time monitoring of endocytosis and trafficking.
- Yoon Namkung
- , Christian Le Gouill
- & Stéphane A. Laporte
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| Open AccessA luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging
D-luciferin is the standard bioluminescent substrate for in vitro and in vivo imaging. Here the authors introduce AkaLumine-HCl, a soluble luciferin analogue with a near-infrared emission maximum, which allows deep tissue imaging at lower concentrations than D-luciferin.
- Takahiro Kuchimaru
- , Satoshi Iwano
- & Shinae Kizaka-Kondoh
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Visualizing the replication of respiratory syncytial virus in cells and in living mice
Respiratory syncytial virus (RSV) causes severe respiratory disease in young children. Here, Rameix-Welti et al. create fluorescent and bioluminescent RSV strains that allow real-time analysis of viral replication and screening of antiviral compounds in cultured cells and in live animals.
- Marie-Anne Rameix-Welti
- , Ronan Le Goffic
- & Jean-François Eléouët
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| Open AccessVisualizing influenza virus infection in living mice
Mouse models of influenza infection that permit monitoring of infection in living animals are scarce. Here Pan et al. describe an engineered influenza virus expressing luciferase, which enables real-time in vivovisualization of viral infection and assessment of antiviral drugs.
- Weiqi Pan
- , Zhenyuan Dong
- & Ling Chen
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| Open AccessLuminescent proteins for high-speed single-cell and whole-body imaging
Luminescent proteins are important tools for biomedical imaging but tend to emit fairly little light. Saito et al.. describe a brighter version of a bioluminescent protein that can visualize intracellular dynamics of various signalling molecules with high spatial and temporal resolution.
- Kenta Saito
- , Y-F Chang
- & Takeharu Nagai