Reviews & Analysis

In two novel RNA modification mapping methods, the authors have engineered RNA enzymes and used the enzyme-mediated mutational signatures to map m⁶A and m¹A at single-nucleotide resolution in mammalian RNA.

Focused laser light can manipulate the phase of electrons and solve the long-standing phase contrast conundrum in electron microscopy.

A widely used concept from machine learning is put to use for single-cell analysis

Single objective light-sheet fluorescence microscopes combine the convenience of conventional sample mounting with sensitive subcellular and super-resolution imaging of cells and tissues.

Understanding of fundamental questions in human embryology is hampered by limited access to in utero developmental events. Manfrin et al. have engineered an in vitro platform that recapitulates early morphogenic events of human development with unprecedented spatial and temporal control.

The interpretation of fragmentation patterns in tandem mass spectrometry is crucial for peptide sequencing, but the relative intensities of these patterns are difficult to predict computationally. Two groups have applied deep neural networks to address this long-standing problem in the proteomics field, extending theoretical spectra with an additional dimension of high-accuracy fragment ion intensities.

Light-sheet fluorescence microscopes are making it possible to follow subcellular dynamics with unprecedented detail, but they are complex to build and maintain. A new method that can generate arbitrary light sheets provides much-needed simplicity and additional versatility.