News & Views in 2014

Two high-throughput single-molecule force spectroscopy platforms expand the reach of this technology for biomechanical molecular phenotyping.

Robots can be used to detect marked animals with less disturbance when assessing ecological drivers of population change.

Protein-responsive ribozymes generated by a streamlined design process will expand the plug-and-play toolbox for synthetic biologists.

Use of adenoviral vectors to deliver donor templates for genome editing facilitates precise genome modifications in human cells. This has implications for both basic and translational applications of rare-cleaving nuclease technologies.

A new distributed computing framework for data analysis enables neuroscientists to meet the computational demands of modern experimental technologies.

Barcoded semisynthetic nucleosomes combined with massively parallel sequencing provide an innovative new platform for analyzing the histone-recognition and histone-modifying activities of chromatin-associated proteins.

A general method to recognize and track unmarked animals within a population will enable new studies of social behavior and individuality.

Experimenter gender affects behavioral assays of pain and stress in laboratory mice.

Two methods are used to identify cis-regulatory sequences by looking at their function.

The combination of mass spectroscopy with immunohistochemistry allows highly multiplexed, directly quantitative imaging of tissue samples for both basic and clinical research.

A first community experiment comparing the performance of analysis methods for single-particle tracking data declares no winner but reveals valuable information for users and developers.

An approach is described that simplifies the isolation of rare human pluripotent stem cells engineered to carry precise disease-relevant mutations.

A new family of genetically encoded ratiometric calcium indicators optimized for imaging of calcium signals in vivo exhibits near-linear fluorescence dynamics while minimizing artifacts caused by movement.