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Current high-throughput 3′ single-cell RNA-sequencing (scRNA-seq) techniques are limited in their ability to elucidate the variable sequences of antigen receptors. Love and colleagues describe a strategy that enables simultaneous analysis of TCR sequences and the corresponding full transcriptomes from 3′-barcoded scRNA-seq samples.
Tissue-resident memory T (TRM) cells have been studied mainly in mouse models. Schumacher and colleagues have developed an imaging technology to track in real time skin-resident human CD8+ TRM cells in situ.
Determining TCR specificity represents a formidable technical barrier to the harnessing of T cell function. Kisielow and colleagues describe a novel platform for efficient determination of TCR specificities in a variety of infectious and cancer settings and in both human systems and mouse systems.
It remains difficult to distinguish cognate APC–T cell interactions in human tissue sections. Clark and colleagues have developed an imaging–machine-learning pipeline that uses deep convolutional and tuned neural networks to identify the combination of distance and cell-shape features that can discriminate between bystander human APC–T cell interactions and cognate interactions in situ.
