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Profiling the epigenetic landscape of the antigen receptor repertoire: the missing epi-immunogenomics data

Nature Methods volume 20, pages 477–481 (2023)Cite this article

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High-resolution sequencing methods that capture the epigenetic landscape within the T cell receptor (TCR) gene loci are pivotal for a fundamental understanding of the epigenetic regulatory mechanisms of the TCR repertoire. In our opinion, filling the gaps in our understanding of the epigenetic mechanisms regulating the TCR repertoire will benefit the development of strategies that can modulate the TCR repertoire composition by leveraging the dynamic nature of epigenetic modifications.

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Fig. 1: Methylation analysis of germline and recombined TCRs.

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Acknowledgements

We acknowledge the funding support for H.A. from the University of Southern California, School of Pharmacy Seed Fund, The Norris Cancer Center pilot fund, STOP Cancer pilot funding, and The Ming Hsieh Institute foundation grants, the NIH P30. H.A. is also supported by NIH-NCI 1R01CA248381–01A1 and in part by NIH grant 5P30CA014089–45.

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Authors and Affiliations

  1. Department of Clinical Pharmacy, School of Pharmacy, University of Southern California, Los Angeles, CA, USA

    Rayyan Aburajab, Mateusz Pospiech & Houda Alachkar

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  1. Rayyan Aburajab
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  2. Mateusz Pospiech
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  3. Houda Alachkar
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Contributions

R.A. researched the literature, and wrote and edited the manuscript. M.P. contributed to writing and editing. H.A. conceived the idea, wrote and edited the manuscript, and supervised the project.

Corresponding author

Correspondence to Houda Alachkar.

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The authors declare no competing interests.

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Nature Methods thanks Grégoire Lauvau and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Aburajab, R., Pospiech, M. & Alachkar, H. Profiling the epigenetic landscape of the antigen receptor repertoire: the missing epi-immunogenomics data. Nat Methods 20, 477–481 (2023). https://doi.org/10.1038/s41592-022-01723-9

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