Light-Seq combines high resolution imaging with next generation sequencing of selected cell populations in fixed biological samples. Specifically, microscopically analyzed cells can be subjected to RNA expression profiling while keeping the sample intact for further assays, enabling cellular phenotypes and states to be assessed in the context of the original tissue.
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This is a summary of: Kishi, J. Y. et al. Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing. Nat. Methods https://doi.org/10.1038/s41592-022-01604-1 (2022).
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Light-Seq: from microscopy to transcriptomics and back. Nat Methods 19, 1353–1354 (2022). https://doi.org/10.1038/s41592-022-01608-x
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DOI: https://doi.org/10.1038/s41592-022-01608-x
