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Novel spike-ins for single-cell sequencing enable ground-truth RNA counting

Nature Methods volume 19, pages 530–531 (2022)Cite this article

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Counting of RNA molecules using unique molecular identifiers (UMIs) is ubiquitous in single-cell sequencing. Here, we introduce molecular spikes, a new type of RNA spike-ins with in-built UMIs. These versatile molecular spikes have many uses in experimental and computational method development and routine biological applications.

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Fig. 1: Molecular spikes.

References

  1. Ziegenhain, C. et al. Comparative analysis of single-cell RNA sequencing methods. Mol. Cell 65, 631–643 (2017). An article that systematically compared scRNA-seq protocols.

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This is a summary of: Ziegenhain, C., Hendriks, G.-J., Hagemann-Jensen, M. & Sandberg, R. Molecular spikes: a gold standard for single-cell RNA counting. Nat. Methods https://doi.org/10.1038/s41592-022-01446-x (2022).

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Novel spike-ins for single-cell sequencing enable ground-truth RNA counting. Nat Methods 19, 530–531 (2022). https://doi.org/10.1038/s41592-022-01456-9

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