We describe a system for introducing guide RNAs (gRNAs) to Cas9-expressing hematopoietic stem cells, which are used to generate mice with gene knockouts in the immune system. By using different gRNA-containing vectors and Cas9-expressing mice, we created systems for knockout of single genes or pairs of genes constitutively or inducibly.
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References
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This is a summary of: LaFleur, M. W. et al. X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes in the immune system. Nat. Immunol. https://doi.org/10.1038/s41590-023-01689-6 (2023).
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A CRISPR-based system for temporal, combinatorial gene knockout in mice. Nat Immunol 25, 15–16 (2024). https://doi.org/10.1038/s41590-023-01694-9
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DOI: https://doi.org/10.1038/s41590-023-01694-9
