We have used human-derived stem cells to generate various microglial states and investigate the function of human microglia in a scalable manner. We were able to model disease-associated microglia that replicated transcriptional signatures found in human tissue, and further developed a lentiviral transfer system to manipulate human microglial states in vitro.
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This is a summary of: Dolan, M.-J. et al. Exposure of iPSC-derived human microglia to brain substrates enables the generation and manipulation of diverse transcriptional states in vitro. Nat. Immunol. https://doi.org/10.1038/s41590-023-01558-2 (2023).
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A toolbox for studying the transcriptional diversity and functions of human microglia in vitro. Nat Immunol 24, 1226–1227 (2023). https://doi.org/10.1038/s41590-023-01570-6
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DOI: https://doi.org/10.1038/s41590-023-01570-6
