Abstract
To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)–somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
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Acknowledgements
We thank M. Evans for providing the HM1 ESC line, T. Shiroishi for providing the JF1 mice, L. Lefebvre for the pROSA26-pA and pROSA26-5′, A. McLaren and J. Ainscough for critical reading of the manuscript, and Y. Kurose for technical assistance.
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The authors declare no competing financial interests.
Supplementary information
Supplementary Fig. 1
Chromosome elimination from CEC11 and CEC12 hybrid cells.
Supplementary Fig. 2
Karyotype of post-Cre CEC11 and CEC12 hybrid cells.
Supplementary Fig. 3
Targeted elimination of ESC-derived chromosome 6s.
Supplementary Fig. 4
Pluripotency of post-Cre CEC6tg/tg hybrid cells.
Supplementary Table 1
Chromosome elimination frequency.
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Matsumura, H., Tada, M., Otsuji, T. et al. Targeted chromosome elimination from ES-somatic hybrid cells. Nat Methods 4, 23–25 (2007). https://doi.org/10.1038/nmeth973
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DOI: https://doi.org/10.1038/nmeth973
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