Abstract
To truly understand the biochemistry of cellular systems, the concentrations of the endogenous protein players must be known, and these can be obtained by careful quantification of fluorescent fusion proteins.
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References
Wu, J.Q. & Pollard, T.D. Counting cytokinesis proteins globally and locally in fission yeast. Science 310, 310–314 (2005).
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Doerr, A. Teaching cell biologists how to count. Nat Methods 2, 892–893 (2005). https://doi.org/10.1038/nmeth1205-892b
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DOI: https://doi.org/10.1038/nmeth1205-892b