The nonphototoxic nature of GFP makes it an excellent imaging probe but a poor tool for techniques that rely on generation of toxic radicals such as chromophore-assisted light inactivation (CALI). Multiphoton excitation helps overcome these limitations.
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Tour, O. EGFP as your targeted 'hitman'. Nat Methods 2, 491–492 (2005). https://doi.org/10.1038/nmeth0705-491
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DOI: https://doi.org/10.1038/nmeth0705-491
