A new method that barcodes cells by assigning different intensities of fluorescent dyes to differently treated samples allows the simultaneous processing of a large number of samples, as one pool, thus increasing the throughput of multidimensional cytometry.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Krutzik, P.O. & Nolan, G.P. Nat. Methods 3, 361–368 (2006).
Waggoner, A. Curr. Opin. Chem. Biol. 10, 62–66 (2006).
Perfetto, S.P., Chattopadhyay, P.K. & Roederer, M. Nat. Rev. Immunol. 4, 648–655 (2004).
Betts, M.R. et al. Blood (in the press).
Mandell, J.W. Am. J. Pathol. 163, 1687–1698 (2003).
Nagata, K., Izawa, I. & Inagaki, M. Genes Cells 6, 653–664 (2001).
Fleisher, T.A. et al. Clin. Immunol. 90, 425–430 (1999).
Chow, S., Patel, H. & Hedley, D.W. Cytometry 46, 72–78 (2001).
Perez, O.D. & Nolan, G.P. Nat. Biotechnol. 20, 155–162 (2002).
Irish, J.M. et al. Cell 118, 217–228 (2004).
Sachs, K., Perez, O., Pe'er, D., Lauffenburger, D.A. & Nolan, G.P. Science 308, 523–529 (2005).
Tong, F.K., Chow, S. & Hedley, D. Cytometry B Clin. Cytom. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jacobberger, J. Increasing the power of cytometry. Nat Methods 3, 343–344 (2006). https://doi.org/10.1038/nmeth0506-343
Issue Date:
DOI: https://doi.org/10.1038/nmeth0506-343
This article is cited by
-
High-content single-cell drug screening with phosphospecific flow cytometry
Nature Chemical Biology (2008)
