Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Six steps closer to FRET-driven structural biology

Nature Methods volume 9, pages 1157–1158 (2012)Cite this article

Subjects

A new toolbox for structural biology that combines single-molecule fluorescence and molecular modeling is used to generate high-precision structures of protein complexes.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: A workflow combining smFRET and computer modeling can provide accurate biomolecular structures.

References

  1. Kalinin, S. et al. Nat. Methods 9, 1218–1225 (2012).

    Article  CAS  Google Scholar 

  2. Stryer, L. & Haugland, R.P. Proc. Natl. Acad. Sci. USA 58, 719–726 (1967).

    Article  CAS  Google Scholar 

  3. Mekler, V. et al. Cell 108, 599–614 (2002).

    Article  CAS  Google Scholar 

  4. Ha, T. et al. Proc. Natl. Acad. Sci. USA 93, 6264–6268 (1996).

    Article  CAS  Google Scholar 

  5. Choi, U.B. et al. Nat. Struct. Mol. Biol. 17, 318–324 (2010).

    Article  CAS  Google Scholar 

  6. Brunger, A.T., Strop, P., Vrljic, M., Chu, S. & Weninger, K.R. J. Struct. Biol. 173, 497–505 (2011).

    Article  CAS  Google Scholar 

  7. McCann, J.J., Zheng, L., Chiantia, S. & Bowen, M.E. Structure 19, 810–820 (2011).

    Article  CAS  Google Scholar 

  8. Muschielok, A. et al. Nat. Methods 5, 965–971 (2008).

    Article  CAS  Google Scholar 

  9. Muschielok, A. & Michaelis, J. J. Phys. Chem. B 115, 11927–11937 (2011).

    Article  CAS  Google Scholar 

  10. Sisamakis, E., Valeri, A., Kalinin, S., Rothwell, P.J. & Seidel, C.A.M. Methods Enzymol. 475, 455–514 (2010).

    Article  CAS  Google Scholar 

  11. Liu, C.C. & Schultz, P.G. Annu. Rev. Biochem. 79, 413–444 (2010).

    Article  CAS  Google Scholar 

  12. Sakon, J.J. & Weninger, K.R. Nat. Methods 7, 203–205 (2010).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physics, Timothy D. Craggs and Achillefs N. Kapanidis are in the Biological Physics Research Group, Clarendon Laboratory, University of Oxford, Oxford, UK,

    Timothy D Craggs & Achillefs N Kapanidis

Authors
  1. Timothy D Craggs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Achillefs N Kapanidis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Timothy D Craggs or Achillefs N Kapanidis.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Craggs, T., Kapanidis, A. Six steps closer to FRET-driven structural biology. Nat Methods 9, 1157–1158 (2012). https://doi.org/10.1038/nmeth.2257

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.2257

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing