Adam, V. et al. Chem. Biol. 18, 1241–1251 (2011).

Increased interest in super-resolution microscopy methods that require fluorophore switching is stimulating the development of more varieties of photoactivatable and photoswitchable fluorescent proteins. Recently, tetrameric and monomeric IrisFP fluorescent proteins that combine the properties of both irreversible green-to-red photoconversion and reversible photoswitching of both colors were reported. Adam et al. report a new member of this fluorescent protein family called NijiFP that displays both types of phototransformations and has some improved characteristics. They demonstrate the flexibility afforded by the multiple phototransformations available and perform photoactivated localization microscopy using both the red and green forms of the protein. This new member of this flexible class of fluorescent proteins should help stimulate creative labeling and imaging applications by biologists.